Not known Factual Statements About thr77
Not known Factual Statements About thr77
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in many tumors, which results in chromosome instability, and thus may add to tumorigenesis. This protein was observed to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two further splice variants were described but in depth nucleotide sequence information will not be however out there.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide normally incorporates the epitope recognized with the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...
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This manual outlines more probable optimization of mobile and immunoassay parameters to make sure the best possible success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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